SDS-PAGE
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a technique that is used to separate proteins in a mixture for the purpose of identifying them individually. Proteins coated in SDS (which gives them a negative charge), are pulled through a thin gel by an electrical current. They separate on the gel based on size--the larger the protein, the slower it moves. Proteins can be stained with Coomassie Blue Dye for visualization or they can be detected by the Western Blotting technique.
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