Xiaoying Lu
Ph.D. in Molecular Biology/Genetics, Sichuan University, Chengdu, Sichuan province, P. R. China, 1999- 2002; Mentor: Chaowu Zhang. Thesis : Studies on Construction and Expression of ß -galactosidase genetically engineered Lactococcus lactis
M.S. in Dietetics, West China University of Medical Sciences, Chengdu, Sichuan province, P. R. China, 1996-1999; Mentor : Linfu Zeng. Thesis : The in vitro Inhibitory Effects of Acanthopanax Giraldii Harms Var. Hispidus Hoo Plant Polysaccharides on Human Gastric Cancer Cell Line and the Molecular Mechanism of Inducing Apoptosis.
M.D. in Medical Science, Baotou Medical College, Baotou City, Inner Mongolia Municipality, P. R. China, 1990-1995.
Research Assistant III Aug 1-current; Department of Speech Pathology and Audiology, University of Iowa. PI : Ingo Titze. Project : Research toward occupational safety in vocalization. This research addresses an important public need, the protection of an estimated 10 million workers in the U.S. who rely heavily on their voice as a primary tool of trade. Evidence has been growing that occupational voice users, such as teachers, telephone workers, ministers, counselors, interviewers, are at risk for vocal injury because they get inadequate recovery times from prolonged speaking. The underlying hypothesis is that there is a limited vibration dose that vocal fold tissues can withstand. Skills: confucal microscopy; H & E staining; cell culture
Postdoctoral Associate March 2006-Jul 31; Dows research institute, University of Iowa. PI : Kim A Brogden. Project : Defensin-induced adaptive immunity to HagB. The severity of periodontal disease is dependent on a combination of host, microbial agent, and environmental factors. One strong correlate related to periodontal disease pathogenesis is the immune status of the host. Our approach to improve the immune status is to use human neutrophil peptide defensins or human beta-defensins, elements of innate host defense, to initiate and regulate the adaptive immune response to foreign antigens. Skills: mice handling; Luminex; Radial diffusion; bone marrow culture; cell culture; ELISA; HPLC; MALDI.
Postdoctoral Associate Jan 2002-Feb 2006; Department of Anatomy and Cell Biology, University of Iowa. PI : Masataka Kawai. Project : To document the molecular mechanisms of contraction in cardiac muscle fibers, in particular to characterize the elementary molecular steps of the cross-bridge cycle in myocardium. Three papers are published in the Journal of Physiology . One paper is published in the Biophysical Journal . One manuscript has been submitted to Journal of Physiology. Skills: Rabbit handling; Purification of protein; Alkali-Urea gel; SDS-PAGE; Muscle dissection; Thin filament reconstitution; Sinusoidal analysis; Photoshop; Excel. Others: Computer skills including the use of software for statistics; Analysis of data and preparation of data and graphs using Excel, NIH Image, Adobe Photo, and Power Point for presentations and papers on PC; Training of research assistants with basic molecular biology experiments and trouble shooting; Maintenance of lab instruments.
Research fellow Jul 1999-Jun 2002; the Institute of Military Medical Science , Kunming , P. R. China. Research on food hygiene and drinking water hygiene and the effective methods to improve the hygiene on food and drinking water.
Physician Jul 1995-Sep 1996; the Affiliated Hospital of Yijinhuoluo Prison, Yijinhuoluo, Inner Mongolia Municipality , P. R. China. General Clinic diseases in the Department of Internal Medicine.
Jan. 2005 to May 2005 Teaching assistant for Histology at the Carver College of Medicine at University of Iowa
Jan. 2000 to Jan. 2002 Instructor for Clinical Biochemistry at Sichuan University
• American Heart Association (AHA) National Scientist Development Grant # 0635360N (USA , 2006-2009; rejected)
• American Heart Association (AHA) postdoctoral fellowship # 0520084Z (USA, 2005)
• American Heart Association (AHA) postdoctoral fellowship # 0320083Z (USA, 2003-2004)
• Biophysical Society 2005 Student Research Achievement Award at the 49th Annual Meeting, February 12-16, 2005, Long Beach, California
• Outstanding PhD thesis Award from Sichuan University (2nd place) (China, 2004)
• Award for Science and Technology Advancement from the People's Liberation Army of China (China, 2001)
• Excellent Ph.D Graduate Award (China, 2001)
• Excellent Undergraduate Award (China, 1992)
Grants
• Natural Science Fund of Sichuan Province , No. 000077 (China , Jan. 1, 2000-Dec. 31, 2002) (Co Investigator)
• Fund of the Institute of Military Medical Science, Chengdu Military Command (China , 2000-2002) (Principal Investigator)
Publications/Presentations
Books
Pingel, L., Lu, X ., and Brogden, K. A. Antimicrobial peptides as mucosal adjuvants. In Virulence Mechanisms of Bacterial Pathogens , 4 th Edition. Brogden, K. A., Stanton , T. B., Cornick, N., Minion, F. C., Zhang, Q, Nolan, L., Wannemuehler, M. (eds.). ASM Press, Washington , DC . To be published 2007.
Reviews
Lu, X ., Kurago, Z. and Brogden, K. A. 2006. Effects of polymicrobial communities on host immunity and response. FEMS Microbiology Letters . Vol. 265 No. 2:141-50.
Research papers
• Lu, X ., Pingel, L. C., Burnell, K. K.,Cavanaugh, J. E., and Brogden, K. A. 2007. Carbamoylcholine chloride induces a rapid elevated IL-6 response in the nasal cavity of C57BL/6 mice. Comparative Medicine . Vol. 57 No. 4: 350-355
• Lu, X ., Tobacman, LS., Kawai, M. 2006. Temperature-dependence of isometric tension and the cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutant. Biophys J . Vol. 91 No. 11: 4230-40
• Lu, X ., Bryant, MK., Keith B., Rubenstein , PA. , Kawai, M. 2005. The role of the N-terminal negative charge of actin in the cross-bridge kinetics and tension generation in reconstituted bovine myocardium. Journal of Physiology, Vol. 564 No. 1: 65-82
• Lu, X ., Tobacman, L. S., Kawai, M. 2003. Effects of tropomyosin internal deletion on isometric tension and the cross-bridge kinetics in bovine myocardium. Journal of Physiology , Vol. 553 No. 2: 457-471
• Fujita, H., Lu, X ., Suzuki, M, Ishiwata, S., Kawai, M. 2004. Effects of tropomyosin and troponin on the elementary steps of the cross-bridge cycle. Journal of Physiology, Vol. 556 No. 2: 637-649.
• Lu, X ., Zhang, C., Pei, X., Liu, X., Yu, Q., Liu, H. 2004. Construction and expression of ß-galactosidase genetically engineered Lactococcus lactis . Journal of Microbiology and Immunology , Vol. 2 No. 4: 243-249
• Lu, X ., Su, M., Li, Y., Zeng L., Liu, X., Li, J., Zheng B., Wang S. 2002. Effect of Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides on the human gastric cancer cell line SGC-7901 and its possible mechanism. Chinese Medical Journal , Vol. 115 No. 5: 716-721.
• Lu, X . 2001. The in vitro Inhibitory Effects of Acanthopanax Giraldii Harms Var. Hispidus Hoo Plant Polysaccharides on Human Gastric Cancer Cell Line and the Molecular Mechanism of Inducing Apoptosis, Carcinogenesis, Teratogenesis and Mutagenesis . Vol. 13 No. 4: 233-234
• Lu, X ., Zeng, L., Li, Y., Wang, W. and Wang, S. 2000. The Inhibitory Effects of Polysaccharide from Acanthopanax giraldii Harms var. Hispidus Hoo on Human Gastric Cancer Cell Line in vitro . Acta Acacemiae Medicinae Militaris Tertiae , Vol. 22 No. 7: 627-630
• Lu, X., Zeng L., Zheng, B., Liu, X., Zheng K. 2000. Inducing Apoptosis Effect of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on Gastric Cancer Cells. Journal of Fourth Military Medical University . Vol. 21, No. 11: 1325 -1326
• Lu, X., Liu, X., Li, Y., Zheng, B., Li, J., Wang, S., Zeng, L. 2001. Inducing Apoptosis Effect of Acanthopanax Garildii Polysacchrides on the Human Gastric Cancer Cell. China Oncology. Vol. 11, No. 1: 137 -139, 142
• Lu, X ., Li, Y., Sun, J., Wang, S., Wang, W., Zeng, L. 2001. Gastric Cancer Cell Apoptosis Induced by Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides in Vitro . The Practical Journal of Cancer , 16: 006-008
• Lu, X ., Zeng L., Wang, S., Wang, W. and Zheng, B. 2000. Inhibiting Effects of Acanthopanax Giraldii Harms Var. Hispidus Hoo Polysaccharides on Human Gastric Cancer Cell Line in Vitro. The Practical Journal of Cancer, Vol. 15 No. 3: 243-245
• Lu, X ., Ma, D., Li, Y., Liu, X., Zheng B., Li, J. and Zeng L. 2001. Effects of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on Oncogenes and Cell factor of Gastric Cancer Cell. Journal of Practical Oncology , Vol.16 No. 1: 45-46
• Lu, X ., 2001. The Inhibitory Character and the Mechanism of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on the in Vitro Human Gastric Cancer Cell Line. Cancer Research on Prevention and Treatment , Vol. 28, No. 4: 256-258
• Lu, X ., Zeng, L., Yang, P., Li, Y., Wang, W., Xiao, N. 2000. Apoptosis of Gastric Cancer Cells Induced by AGP. Chinese New Drugs Journal , Vol. 9 No. 3: 166-167
• Lu, X., Wang, W., Zheng, K. 2000. Studies on the Biological Effect of Acanthopanax Polysaccharides. Chinese Journal of Information of Traditional Chinese Medicine. Vol. 7, No. 6: 46 -47
• Lu, X ., Zhang, C., Pei, X., Yu, Q. and Song, Y. 2001, A Study on Main Character of Three Lactic Acid Bacteria as Vector-host System. Chinese Journal of Microecology , Vol 13 No. 5: 265-266
• Lu, X. , Su, M. Zeng, L., Liu, X., Zheng, K., Zheng, B. 2000. Inhibitory character of Acanthopanax Giraldii Harms Var. Hispidus Hoo Polysaccharides on the in Vitro Human Gastric Cancer Cell. China Public Health , Vol. 16 No. 11: 1005-1006
• Lu, X., Zeng, L., Cai, Y., Li, Y., Liu, X. 2002. The Apoptosis of Human Gastric Cancer Cell Induced by Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides in Vitro . Journal of West China University of Medical Sciences. Vol. 33, No. 2: 265-266, 273
• Lu, X. , Zeng, L., Su, M., Liu X., Yang P. 2000. The Inhibitory Effect of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on Proliferation of Gastric Cancer Cell Line and Its Mechanism. Journal of West China University of Medical Sciences, Vol. 31 No. 4: 478-480
• Lu, X., Zhang, C. 2003. Recent Studies on Lactococcus Gene Clone Vector System. Modern Preventive Medicine. Vol. 30, No. 2: 222 -225
• Lu, X., Zhang, C. 2003. Studies on Lactococcus Gene Expression Host-Vector System. Modern Preventive Medicine. Vol. 30, No. 1: 53 -55
• Lu, X ., Zhang, C., Yu, Q., Yao , X., Pei , X., Liu, H. 2002. Selection of High Activity ß-galactosidase Bulgaricus Lactobacillus . Modern Preventive Medicine, Vol. 29, No. 6: 756-759
• Lu, X., Zeng, L., Li, Y., Su, M., Zhang, L., Liu X. 2000. The Inhibitory Effect of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on the Stem Cell of Gastric Cancer. Modern Preventive Medicine. Vol. 27, No. 4: 453 -454
• Lu, X ., Zeng, L., Liu X. and Li, J. 2000. The Inhibitory Effects of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on the in vitro Human Gastric Cancer Cell Line. Modern Preventive Medicine , Vol. 27 No. 2: 210-211
• Lu, X . 1998. The Effect of Ganoderma Lucidum Tea on Life Span of the Drosophila Melanogaster. Modern Preventive Medicine , Vol. 25, No. 3: 381-382 .
• Lu, X., Wu, J., Wang, S. 2000. Mechanism of Anticancer Effect of Acanthopanax Polysaccharides. Journal of Preventive Medicine Information. Vol. 16, No. 3: 214 -216
• Lu, X ., Zeng, L., Wu, J., Wu, H., Zheng, K. 2000. Inhibitory Effect of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on the Proliferation of Human Gastric Cancer Cell in Vitro . Journal of Preventive Medicine Information, Vol. 16, No. 1: 13-14
• Lu, X., Liu X. 2000. Progress of Gastric Cancer Related Genes. Foreign Medical Sciences supplement . Vol. 27: 196-197
• Lu, X., Wang, S., Zheng, K. Cell Apoptosis and Gastric Cancer. Tumor basic and clinical Research. 158-159
• Lu, X., Zhang, C. 2001. Migratory Birds and Spread of West Nile Virus in the Western Hemisphere . Modern Preventive Medicine. Vol. 28, No. 2: 235-240 (Translation).
• Su, Q., Lu, X., Zhang, C. 2005. Establishment of in vitro toxic coca-2 cell model caused by lactose. Modern Preventive Medicine , Vol. 32 No. 12: 1620-1621
• Liu, X., Lu, X., Yu, Q., Zhang, C. 2005. Relief effect of beta-galactosidase genetically engineered Lactococcus lactis on the cell toxicity caused by lactose. Journal of Sichuan University (Medical science edition) , Vol. 37 No. 1: 52-54
• Su, M, Dai, P. Lu, X., Wan, B. Li, H., Liu, J. 2001. Changes of genes and cell factors of the human gastric cancer cell SGC-7901 by Aining. Modern Preventive Medicine , Vol. 28 No. 1: 6-7
• Su, M., Dai, M., Lu, X. , Li, H., Liu, J. 2002. Effect of Traditional Medicine Compounds Aining on the Expression of Apoptosis Inducing Genes of Human Gastric Cancer Cell. Journal of Chinese Medicinal Materials , Vol. 25 No. 8: 563-566
• Su, M., Dai, M., Lu, X. , Li, H., Liu, J. 2002. Morphological Changes and Inhibiting Effect on Human Gastric Cancer Cell SGC-7901 Caused by Aining. Journal of Chinese Medicinal Materials , Vol. 25 No. 5: 339-342
• Su, M., Dai, M., Lu, X. , Liu J., Li, H. 2002. Apoptotic Morphological Changes and TUNEL of Human Gastric Cancer Cell SGC-7901 Induced by Aining. Journal of Chinese Medicinal Materials . Vol. 25 No. 4: 270-272
• Su, M., Lu, X. , Li, H., Wan B., Liu J. 2001. Study on Apoptosis of Human Gastric Adenocarcinoma Cell SGC-7901 Induced by Aining in vitro and the cell cycle. Journal of Chinese Medicinal Materials , Vol. 24 No. 12: 876-879
• Wang, C., Zhang, C., Yu, Q., Pei, X., Lu, X. 2003. Characterization of One Lactobacillus and Its Lactase Activity. Chinese Journal of Public Health. Vol. 19, No. 10: 1276-1277
• Wang, C., Zhang, C., Pei, X., Yu, Q., Lu, X. 2004. Amplification of Lactobacillus bulgaricus ß-galactosidase gene and optimization of the reaction condition. Journal of Sichuan University (Medical Science Edition). Vol. 35, No. 2: 217-219
• Wang, C., Zhang, C., Du, J., Lu, X. 2005. Study of the genetic stability of expression plasmid vector pMG36e in host bacteria Wei Sheng Yan Jiu 34 (2) : 214-216
Proceedings
Lu, X ., Pingel, L., Hansen, C., Burnell, K. K., and Brogden, K. A. Defensins Dampen the Mucosal Cytokine Response to Microbial Antigens. Proceedings, 57th American College of Veterinary Pathologists Annual Meeting, December 2-6, 2006, Tucson , Arizona .
Abstracts
• Lu, X ., Kurago, Z. B., Pingel, L.C., Burnell, K. K., Belanger, M., Progulske-Fox, A., and Brogden, K. A. Defensin-altered cytokines by murine dendritic cells to Porphyromonas gingivalis HagB. 85 th General Session IADR meeting, New Orleans , LA. March 21-24, 2007.
• Lu, X ., Kurago, Z. B., Pingel, L.C., Burnell, K. K., Belanger, M., Progulske-Fox, A., And Brogden, K. A. a - and b -Defensins alter cytokine responses of murine dendritic cells to Porphyromonas gingivalis hemagglutinin B. Virulence Mechanisms of Bacterial Pathogens, International Symposium, Ames , IA , September 6-8, 2006
• Pingel, L., Burnell, K.K., Lu, X ., Belanger, M., Progulske-Fox, A., Kurago, Z., and Brogden, K. A. Defensins suppress cytokine response in human dendritic cells to Porphyromonas gingivalis hemagglutinin B. 85 th General Session IADR meeting, New Orleans , LA. March 21-24, 2007.
• Kawai, M., Stanton, K.J., Lu, X ., Hitchcock-DeGregori, S.E. Period 3 of tropomyosin is essential to enhance isometric tension in the thin-filament reconstituted cardiac muscle fibers. Submitted to Biophysical Society 51st Annual Meeting, March 3-7, 2007. Baltimore , Maryland .
• Lu, X., Heeley, D.H., Smillie, L.B., Kawai, M. 2006. Effect of Tropomyosin (Tm) on Isometric Tension and Cross-bridge Kinetics in Bovine Myocardium. Biophysical Journal. Vol. 90 No. 1: 120a .
• Lu, X., Fraundorf, L., Kawai, M. 2006. Effect of polyethylene glycol (PEG) on force, and elementary steps of the cross-bridge cycle in rabbit psoas muscle fibers. Biophysical Journal. Vol. 90 No. 1: 569a .
• Lu, X., Tobacman, L.S. Kawai, M. 2005. Tropomyosin influences actomyosin interface to enhance hydrophobic and stereospecific interactions in bovine myocardium. Biophysical Journal. Vol. 88 No. 1: 359a.
• Lu, X ., Rubenstein, PA., Kawai, M. 2005. Negative charges at the N-terminus of actin modify actomyosin interface and force generation capacity in reconstituted bovine myocardium. Biophysical Journal. Vol. 88 No. 1: 129a.
• Kawai, M., Lu, X., Rubenstein, P.A. 2004. The number of the N-terminal negative charges of actin modifies the amount of force generated per cross-bridge in reconstituted bovine cardiac muscle fibres. Journal of Muscle Research and Cell Motility. Vol. 26, No. 1: 64
• Kawai, M., Lu, X. , Rubenstein, P.A. 2004. The N-terminal negative charges of actin modify actomyosin interface, the ATP binding site, and the capacity to generate force in reconstituted cardiac muscle fibers. Journal of Muscle Research and Cell Motility. Vol. 25, No. 3:259
• Lu, X., Tobacman, L.S. Kawai, M. 2004. Tropomyosin modifies actomyosin interface to enhance stereospecific and hydrophobic interaction and to increase force in myocardium. Journal of Muscle Research and Cell Motility. Vol. 25, No. 3: 247
• Lu, X ., Bryant, MK., Rubenstein, PA., Kawai, M. 2004. The role of the N-terminal Negative Charge of Actin in the Cross-bridge Kinetics and Tension Generation in Reconstituted Bovine Myocardium. Biophysical Journal . Vol. 86 No. 1 (supplement): 118a.
• Lu, X ., Tobacman, L. S., Kawai, M. 2004. The Temperature Effect of Tropomyosin Internal Deletion (?23Tm) on Isometric Tension and the Cross-bridge Kinetics in Bovine Myocardium. Biophysical Journal . Vol. 86 No. 1 (supplement): 216a.
• Lu, X ., Tobacman, L. S., Kawai, M. 2003. Effects of tropomyosin (Tm) internal deletions on isometric tension and the cross-bridge kinetics in bovine myocardium. Biophysical Journal . Vol. 84 No. 2: 569a
• Fujita, H., Lu, X ., Suzuki, M., Ishiwata, S., Kawai, M. 2003. Effect of tropomyosin (Tm) on active tension and elementary steps of the cross-bridge cycle. Biophysical Journal . Vol. 84 No. 2: 569a-570a
Conference Posters
• Tropomyosin influences actomyosin interface to enhance hydrophobic and stereospecific interactions in bovine myocardium. July 2005. Gordon research conferences. New London , NH .
• N-terminal negative charges of actin modify actomyosin interface and capacity to generate force in reconstituted cardiac muscle fibers. July 2005. Gordon research conferences. New London , NH .
• Negative charges at the N-terminus of actin modify actomyosin interface and force generation capacity in reconstituted bovine myocardium. Feb 2005. 49 th Biophysical society meeting. Long Beach , CA .
• The Temperature Effect of Tropomyosin Internal Deletion (?23Tm) on Isometric Tension and the Cross-bridge Kinetics in Bovine Myocardium. Feb 2004. 48 th Biophysical society meeting . Baltimore , MD.
• Effects of tropomyosin (Tm) internal deletions on isometric tension and the cross-bridge kinetics in bovine myocardium. Mar 5, 2003. 47 th Biophysical society meeting. San Antonio , TX .
• Effect of tropomyosin (Tm) on active tension and elementary steps of the cross-bridge cycle. Mar 5, 2003. 47 th Biophysical society meeting. San Antonio , TX .
• Effects of ?23Tm on isometric tension and the cross-bridge kinetics in bovine myocardium. Apr 1, 2003. University of Iowa . Iowa City , IA.
• Effects of tropomyosin mutant on isometric tension and the cross-bridge kinetics in bovine myocardium. Feb 2003. Department of Anatomy and Cell Biology, University of Iowa . Iowa City , IA.
• Period 3 of tropomyosin is essential to enhance isometric tension in the thin-filament reconstituted cardiac muscle fibers. Apr 4, 2007. University of Iowa . Iowa City , IA.
Seminars
• Effects of tropomyosin (Tm) internal deletions on isometric tension and the cross-bridge kinetics in bovine myocardium. Oct 23, 2003. Department of Anatomy and Cell Biology, University of Iowa .
• A study of ß-galactosidase of genetically engineered Lactococcus lactis . May 14, 2002. Department of Anatomy and Cell Biology, University of Iowa .
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